| Public Sector |
| 1 | College of Nursing (F) (Govt.) Larkana. | |
| 2 | College of Nursing (M) (Govt.) Larkana. | |
| 3 | College of Nursing (M) (Govt.) Jacobabad. | |
| 4 | College of Nursing (F) (Govt.) Khairpur. | |
| 5 | College of Nursing (F) (Govt.) R.B.U.T. Shikarpur. | |
| 6 | College of Nursing (F) (Govt.) Sukkur. | |
| Private Sector |
| 7 | Lareb Mustafa Institute of Nursing and Allied Health Sciences, Gambat. | |
| 8 | Sindhu Institute of Nursing, Khairpur Mir's. | |
| 9 | Imam Institute of Nursing and Allied Health Sciences, Jacobabad. | |
| 10 | Zaib-un-Nisa Institute of Nursing and Allied Health Sciences, Ghotki. | |
| 11 | Moomal Institute of Physiotherapy and Allied Health Sciences, Hyderabad. | |
| 12 | SHAAF Institute of Modern Sciences, Larkana. | |
| 13 | Duaa Institute Medical & Allied Health Sciences, Hyderabad. | De-affiliated w.e.f. 2023 |
| 14 | Illahi Bux Institute of Nursing, Nawabshah, SBA. | |
| 15 | Bhittai Institute of Nursing, Nawabshah, SBA. | |
| 16 | Shaheed Molai Khan Institute of Nursing, Shikarpur. | |
| 17 | National Institute of Allied Health Sciences, Qasimabad, Hyderabad. | |
| 18 | Sindh Institute of Health and Technology, Khairpur, Sindh. | |
| 19 | Leaders Institute of Medical and Health Sciences, Khairpur. | |
| 20 | Visionary Institute of Nursing and Allied Health Sciences, Sukkur. | |
| 21 | Manzoor Institute of Nursing & Allied Health Sciences, Jacobabad. | |
| 22 | Adam College of Nursing and Midwifery, Hyderabad. | |
| 23 | Larkana Institute of Nursing and Allied Health Sciences, Larkana. | |
| 24 | Institute of Medical & Technology (IMT), Sukkur. | |
| 25 | Mehmood Institute of Nursing and Allied Health Sciences, Sanghar. | |
| 26 | Sindh Institute of Cardiovascular Diseases, Sukkur. | |
| 27 | Sindh Institute of Cardiovascular Diseases, Larkana. | |
| 28 | EduCareer Sindh Institute (Pvt.) Ltd., Sukkur. | |
| 29 | Rohri Institute of Sukkur & Allied Health Sciences, Rohri. | |
| 30 | Nova Institute of Modern Studies (Pvt.) Ltd., Islamabad. | De-affiliated 2023 |
| 31 | Larkana Institute of Health & Emerging Sciences, Larkana. | De-affiliated 2024 |
| 32 | Bhittai College of Nursing & Allied Health Sciences, Mirpur Khas. | De-affiliated 2022 |